Figure 2.

Activation of HNF4α gene by CRISPR/dCas9-VP64. (A) Schematic representation of the lentivirus expressing HNF4α sgRNA; (B) schematic representative of the lentivirus expressing dCas9; (C) sequencing result of the HNF4α sgRNA vector construction; (D) illustration of the CRISPR/dCas9-VP64 mediated activation of HNF4α; (E) expression of HNF4α mRNA in HSCs treated as indicated was analyzed by qPCR. Negative control served as NC. Data are expressed as mean ± SEM of three different experiments. **p < .01.