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. 2020 Dec 18;28(1):10–18. doi: 10.1080/10717544.2020.1850917

Figure 3.

Figure 3.

AML12-Exos efficiently encapsulate CRISPR/dCas9-VP64 system. (A) Schematic representation of the exosomes encapsulation procedure; (B) western blot analysis of the expression of protein dCas9 in the isolated exosomes and the parental cells. GAPDH served as the loading control; (C) expression of dCas9 mRNA in the isolated exosomes and the parental cells was analyzed by qPCR; (D) expression of sgRNA in the isolated exosomes and the parental cells was analyzed by qPCR. Negative control served as NC. Data are expressed as mean ± SEM of three different experiments. ***p < .005.