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. 2020 Dec 18;28(1):10–18. doi: 10.1080/10717544.2020.1850917

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Validation and characterization of AML12 cells and HSCs. (A) Microscope image of the AML12 and HSC cells morphology; (B) western blot analysis of the expression of HNF4α/CRBP1 and α-SMA/collagen I in AML12 cells and HSCs. GAPDH served as the loading control. (C) immunofluorescence microscope image of the expression of HNF4α in the liver tissues and AML12 cells (scale bar = 5 μm). Nuclei were counterstained with Hoechst; (D) immunofluorescence microscope image of the expression of CRBP1 in the liver tissues and HSCs (scale bar = 2.5 μm). Nuclei were counterstained with Hoechst.