Figure 5.

AML12-Exos effectively deliver CRISPR/dCas9-VP64 system into HSCs in vitro. (A) Schematic representation of the exosomes endocytosis procedure by the recipient cells; (B) immunofluorescence microscope image of the colocation of DiI-labeled exosomes and CRBP1 in HSCs (scale bar = 5 μm). Nuclei were counterstained with Hoechst; (C) immunofluorescence microscope image of the different cells-derived DiI-labeled exosomes endocytosis efficiency by the HSCs (scale bar = 5 μm). Nuclei were counterstained with Hoechst; (D) expression of dCas9 mRNA, sgRNA, HNF4α mRNA in the HSCs treated as indicated was analyzed by qPCR. Data are expressed as mean ± SEM of three different experiments. **p < .01; (E) western blot analysis of the expression of dCas9/HNF4α/E-Cadherin and α-SMA/collagen I in the HSCs treated as indicated. GAPDH served as the loading control.