Figure 2.

ADMSCs and their derived exosomes alleviate H₂O₂-induced HaCaT cell damage. (A) MTT assay was used to measure the effect of different concentrations of H₂O₂ on the viability of HaCaT cells (one-way ANOVA, *P < 0.05 or **P < 0.01 vs 0 µM). (B) Flow cytometry was implemented to measure the effect of different concentrations of H₂O₂ on the apoptosis of HaCaT cells (one-way ANOVA, *P < 0.05 or **P < 0.01 vs 0 µM). (C) Transwell co-culture system. (D) MTT assay was utilized to measure the effect of ADMSCs and exosome treatment on the viability of HaCaT cells treated with H₂O₂ (one-way ANOVA, *P < 0.05 vs the model group; # P < 0.05 vs ADMSCs group). (E) Flow cytometry was performed to measure the effect of ADMSCs and exosome treatment on the apoptosis of HaCaT cells treated with H₂O₂ (one-way ANOVA, *P < 0.05 vs the model group; # P < 0.05 vs ADMSCs group). (F) ELISA was utilized to measure the effect of ADMSCs and exosome treatment on the expression of apoptosis-related genes (C-caspase3, Bcl-2) and inflammation-related genes (IL-6, IL-10) in HaCaT cells treated with H₂O₂ (one-way ANOVA, *P < 0.05 vs the model group; # P < 0.05 vs ADMSCs group). (G) Wound healing test was performed to measure the effect of ADMSCs and exosome treatment on the migration ability of HaCaT cells treated with H₂O₂ (one-way ANOVA, *P < 0.05 vs the model group; # P < 0.05 vs ADMSCs group).