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. 2020 Dec 13;23(2):141. doi: 10.3892/mmr.2020.11780

Figure 2.

Figure 2.

miR-375 interacts with circARRDC3. (A) Bioinformatics prediction of putative binding site at circARRDC3 by miR-375. (B) RT-qPCR was performed to determine the expression of miR-375 in NECs transfected with miR-375 mimic or miR-375 inhibitor. (C) Dual luciferase reporter assay showed luciferase activity in WT circARRDC3-containing NECs transfected with NC mimic, miR-375 mimic, NC inhibitor and miR-375 inhibitor. (D) miR-375 expression levels were detected by RT-qPCR in IL-13-induced NECs transfected with si-NC, si-circARRDC3, and si-circARRDC3 + miR-375 inhibitor. The levels of (E) GM-CSF, (F) eotaxin (G) and MUC5AC were detected by RT-qPCR in IL-13-induced NECs transfected with si-NC, si-circARRDC3, and si-circARRDC3 + miR-375 inhibitor. (H) Cell viability was examined by an MTT assay in IL-13-induced NECs transfected with si-NC, si-circARRDC3, and si-circARRDC3 + miR-375 inhibitor. (I) Cell apoptosis was examined using a TUNEL assay in IL-13-induced NECs transfected with si-NC, si-circARRDC3, si-circARRDC3 + miR-375 inhibitor. Magnification, ×100. *P<0.05. ARRDC3, arrestin domain-containing 3; circ-, circular RNA; NEC, nasal epithelial cell; RT-qPCR, reverse transcription-quantitative PCR; si-, small interfering RNA; NC, negative control; GM-CSF, granulocyte-macrophage colony-stimulating factor; MUC5AC, mucin 5AC; miR, microRNA; WT, wild-type; Mut, mutant.