Figure 1.

BNIP3 expression is downregulated in radioresistant NPC cells. (A) A total of 53 significant DEGs were identified in the GSE48503 dataset between radioresistant NPC samples and radio-sensitive NPC samples. Enrichment analysis was performed on the DEGs. The ‘Foxo signaling pathway’ was shown to be the key pathway associated with BNIP3, CCNG2 and HOMER2. (B) The 5-year survival rates of patients based on CCNG2, BNIP3 and HOMER2 expression, stratified by expression, was analyzed using Kaplan-Meier plotter. (C) 5-8F-R and 5-8F cells were exposed to different doses of IR, and the cell viability was measured. (D) Expression of BNIP3 mRNA was detected in 5-8F-R cells and 5-8F cells following IR. (E) Expression of BNIP3 protein was detected in 5-8F-R cells and 5-8F cells following IR treatment. *P<0.05, **P<0.001 vs. 0 h. DEG, differentially expressed gene; IR, irradiation; NPC, nasopharyngeal carcinoma; BNIP3, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; CCNG2, cyclin-G2; HOMER2, homer protein homolog 2.