Figure 3.

BNIP3 reduces radioresistance of nasopharyngeal carcinoma cells by altering invasion and apoptosis. (A) Transwell invasion assays were used to measure invasion of the transfected 5-8F and 5-8F-R cells treated with 4 Gy IR. (B) Flow cytometry was used to detect the rate of cell apoptosis in the transfected 5-8F and 5-8F-R cells following treatment with 4 Gy IR. Western blotting was used to measure the protein expression levels of (C) invasion-related proteins and (D) apoptosis-related proteins in 5-8F and 5-8F-R cells treated with 4 Gy IR. *P<0.05, **P<0.001 vs. NC. BNIP3, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; NC, negative control; IR, irradiation; si-, small interfering RNA; OE, overexpression.