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. 2020 Dec 10;23(2):130. doi: 10.3892/mmr.2020.11769

Figure 4.

Figure 4.

BNIP3 is the target gene of miR-182-5p, and its expression is reduced by miR-182-5p. (A) TargetScan was used to predict the binding site between BNIP3 and miR-182-5p. (B) A dual-luciferase reporter assay was performed to validate the binding between BNIP3 and miR-182-5p. **P<0.001 vs. WT+NC group. (C) Transfection efficiency of miR-182-5p inhibitor or mimic in 5-8F and 5-8F-R cells. (D) BNIP3 protein expression levels in the 5-8F and 5-8F-R cells transfected with miR-182-5p inhibitor or mimic. *P<0.05, **P<0.001 vs. NC. BNIP3, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; WT, wild-type; MUT, mutant; NC, negative control; miRNA/miR, microRNA; UTR, untranslated region.