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Expression of STAT3, phospho-STAT3, PARP-1, IL-6 and TGF-β1 in colonic tissues. (A) The protein levels of STAT3, phospho-STAT3 and PARP-1 were measured by western blot analysis. GAPDH was used as an internal control for grayscale analyses. (B and C) The mRNA levels of IL-6 and TGF-β1 were measured by RT-qPCR. The results are presented as the means ± SD (n=10). *P<0.05 vs. control; #P<0.05 vs. DSS group.5-AIQ, 5-aminoisoquinolinone; DSS, dextran sodium sulfate.
