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. 2020 Dec 8;23(2):125. doi: 10.3892/mmr.2020.11764

Figure 1.

Figure 1.

Verification of overexpression of BMP2 in hMSCs. (A) Quantitative expression analysis of BMP2 mRNA examined by reverse transcription-quantitative PCR with GAPDH mRNA as an internal reference. Values are normalized to levels observed in cells with control vector (OE-NC, using pcDNA3.1 as overexpression control) (n=3, paired Student's t-test, mean ± SD). (B) Immunoblotting for BMP2 from control (OE-NC) and BMP2-overexpressing hMSCs with GAPDH as a loading control. ***P<0.01 vs. OE-NC. BMP2, bone morphogenetic protein 2; hMSC, human mesenchymal stem cells; OE, overexpression; NC, negative control.