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. 2020 Dec 13;23(2):139. doi: 10.3892/mmr.2020.11778

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Copyright: © Han et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — FLVCR1-AS1 functions as a competing endogenous RNA for miR-381 in CRC. (A) The binding sequence between FLVCR1-AS1 and miR-381 was predicted using starBase. (B) RT-qPCR results show that the level of miR-381 was successfully increased in Caco-2 and SW480 cells transfected with miR-381 mimics. (C) Luciferase reporter assay results showing the luciferase activity of FLVCR1-AS1-wt and FLVCR1-AS1-mut in 293T cells transfected with NC mimics or miR-381 mimics. *P<0.05. (D) RNA pull-down assay was used to verify the interaction between FLVCR1-AS1 and miR-381 in Caco-2 and SW480 cells transfected with bio-miR-381-wt or bio-miR-381-mut. *P<0.05 vs. Bio-miR-NC. (E) RT-qPCR assay results show that the level of miR-381 is lower in CRC tissues compared with normal tissues. (F) RT-qPCR assay results show that the expression of miR-381 is increased in cells transfected with shFLVCR1-AS1. (G) RT-qPCR results show that the level of miR-381 in Caco-2 and SW480 cells was successfully reduced by transfection with miR-381 inhibitor. (H) Cell Counting Kit-8, (I) Transwell migration assay (magnification, ×200) and (J) invasion assay results demonstrating the proliferation, migration and invasion of Caco-2 and SW480 cells transfected with shNC, shFLVCR1-AS1 and shFLVCR1-AS1+ miR-381 inhibitor. *P<0.05. FLVCR1-AS1, feline leukemia virus subgroup C receptor 1 antisense RNA 1; miR, microRNA; CRC, colorectal cancer; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; bio, biotin; wt, wild-type; mut, mutant; sh, short hairpin; NC, negative control; OD450, optical density at 450 nm.