Form of SHEDs changed from circle, fusiform to fibroblasts. Panel (a), the detached single-cell suspension showed the cytoplasm was uniform, bright, smooth and clear, and small in size (×100). Panel (b), the SHEDs were fusiform (×100). Panel (c), the third generation of SHEDs grew in braided, radial and whirlpool colonies, and tended to become longer, they were in the shape of pulp fibroblasts (×100). SHEDs, Stem cells from human exfoliated deciduous teeth