Figure 6.

miR-221 overexpression or CHD8 silencing increased mRNA expression of Wnt/β-catenin pathway-related factors and decreased CHD8 mRNA expression in SHEDs. *, p < 0.05 vs. the mimic-NC group; #, p < 0.05 vs. the inhibitor-NC group; &, p < 0.05 vs. the si-NC group; @, p < 0.05 vs. the miR-221 inhibitor group. Measurement data were expressed as mean ± standard deviation. One-way analysis of variance (ANOVA) was adopted to analyze comparison between multiple groups with Tukey’s post hoc test. The experiment was repeated three times. CHD8, Chromodomain-Helicase-DNA-binding protein 8; SHEDs, stem cells from human exfoliated deciduous teeth; NC, negative control