Figure 7.

miR-221 overexpression or CHD8 silencing increased protein expression of Wnt/β-catenin pathway-related factors and decreased CHD8 protein expression in SHEDs. (a) Gray value of CHD8, β-catenin, Wnt3a, Axin2, c-Myc, Gsk-3β, TH, and phosphorylated Gsk-3β protein bands in SHEDs after transfection determined by Western blot analysis. (b) Protein expression of CHD8, β-catenin, Wnt3a, Axin2, c-Myc, Gsk-3β, TH, and phosphorylated Gsk-3β in SHEDs after transfection determined by Western blot analysis. *, p < 0.05 vs. the mimic-NC group; #, p < 0.05 vs. the inhibitor-NC group; &, p < 0.05 vs. the si-NC group; @, p < 0.05 vs. the miR-221 inhibitor group. Measurement data were expressed as mean ± standard deviation. One-way analysis of variance (ANOVA) was adopted to analyze comparison between multiple groups with Tukey’s post hoc test. The experiment was repeated three times. CHD8: Chromodomain-Helicase-DNA-binding protein 8; Axin2, Axis inhibition protein 2; TH, neurofilament protein; miR-221, microRNA-221; NC, negative control