Figure 5.

ABs improved EC function via inducing TFEB expression. (A) HUVECs were transfected with siRNA targeting TFEB (20 nmol/L) for 72 h, and the expression of TFEB protein was detected by western blots. (B) HUVECs proliferation was detected by MKI67 staining in the control group and in the TFEB knockdown group with or without AB treatment (20 µg/ml). Scale bar: 50 µm. Cell migration assay (C) and tube formation assay (D) were also performed in the control and TFEB knockdown group with or without AB treatment (20 µg/ml). Scale bar: 20 µm. The tube formation assay results were quantified by ImageJ software. The results were representative of data generated in at least 3 independent experiments. The data were presented as mean ± SEM. ns, not significant; **P < 0.01 by Student’s t-test (A) or one-way ANOVA (B, C, D)