Figure 1.

ZIKV infection reduces the KPNA2 protein level. (A) ZIKV infection of Vero cells leads to a lower KPNA2 protein level while having minimal effect on KPNA1. The cells were inoculated with ZIKV PRVABC59 strain at an MOI of 10 and harvested for western blotting (WB) at 24 h post-infection (hpi). Relative levels of KPNA2 are shown below the images after normalization with house-keeping gene TUBB1/tubulin beta. (B) ZIKV infection has minimal effect of KPNA2 mRNA level detected by RT-qPCR. The relative KPNA2 mRNA level is shown in comparison to the mock-infected Vero cells after normalization with the house-keeping gene RPL32. The cells were infected with ZIKV PRVABC59 strain at an MOI of 10 and harvested at 24 hpi. Error bars represent the standard errors of the means of three repeated experiments. (C and D) ZIKV infection of HeLa and SK-N-SH cells reduces KPNA2 level while having minimal effect on KPNA1. The cells were inoculated with ZIKV PRVABC59 strain at an MOI of 10 and harvested at 48 hpi. (E) The infection of Vero cells with incremental ZIKV inoculum leads to a dose-dependent reduction of KPNA2. The cells were harvested for WB at 24 hpi. Relative levels of KPNA2 are shown below the images. (F) ZIKV RNA levels in the infected cells at 24 hpi detected by RT-qPCR. The relative RNA levels in comparison with the MOI of 0.1 are shown. Error bars represent the standard errors of the means of three repeated experiments. Significant differences in RNA level from the sample of MOI 0.1 are denoted by asterisks (**, P < 0.01; ***, P < 0.001). G. ZIKV reduces KPNA2 in Vero cells at both 24 and 48 hpi