Figure 2.

ZIKV infection induces KPNA2 degradation via the lysosomal pathway. (A) MG132 and NH₄Cl treatment restore the KPNA2 level in ZIKV-infected cells. Vero cells were infected with ZIKV and 24 hpi, treated with MG132 or NH₄Cl. The cells were harvested 6 h after the treatment. DMSO was included as a solvent control in the first two lanes. (B) The treatment with MG132 and NH₄Cl for 6 h has minimal effect on cell viability. DMSO was included as a solvent control in the first two bars. (C) The treatment with MG132 and NH₄Cl for 6 h has minimal effect on the ZIKV RNA level. (D) KPNA2 half-life is shortened by ZIKV infection. Vero cells were infected with ZIKV at an MOI of 10 for 24 h before treated with cycloheximide. Mock-infected cells were included for control. (E) ZIKV infection has a minimal effect on KPNA2 ubiquitination. Vero cells were infected with ZIKV, treated with NH₄Cl for 6 h, and harvested for immunoprecipitation (IP) with KPNA2 antibody. The IP precipitate was subjected to WB with antibodies against ubiquitin (Ub) and KPNA2. The input of cell lysate was included as a control