Table 1.
Primera | Sequences (5ʹ to 3ʹ)b | Target gene/vector |
---|---|---|
C-F1 | CGAATTCATGAAAAACCCAAAAAAGAAATC | C |
C-R2 | GCTCGAGTGCCATGGCTGTGGTCAGCAG | C |
PRM-F2 | TGAATTCGCCACCATGGGCACAGATACTAGTGTCGGAAT | prM |
PRM-R2 | GCTCGAGGCTGTATGCCGGGGCAATCAG | prM |
E-F2 | TGAATTCGCCACCATGCAAAAAGTCATATACTTGGTC | E |
E-R2 | GCTCGAGAGCAGAGACGGCTGTGGATAAG | E |
NS2A-F2 | TGAATTCCACCATGGGATCAACTGATCACATGGAC | NS2A |
SPG-C16-NS2A-F1 | CGAATTCGCCACCATGGGAGTCAAAGTTCTGTTTGCCCTGATCTGCATCGCTGTGGCCGAGCCCAGGAAAGAACCAGAAAG | NS2A |
NS2A-R2 | TCTCGAGCCGCTTCCCACTCCTTGTGAG | NS2A |
NS2B-F1 | TGAATTCAGCTGGCCCCCTAGCGAAGTAC | NS2B |
NS2B-R1 | GCTCGAGTCACCTTTTTCCAGTCTTCACG | NS2B |
NS3-F1 | TGAATTCAGTGGTGCTCTATGGGATGTG | NS3 |
NS3-R1 | TCTCGAGTCATCTTTTCCCAGCGGCAAACTC | NS3 |
NS4A-F1 | TGAATTCGGAGCGGCTTTTGGAGTGATG | NS4A |
NS4A-R1 | TCTCGAGTCATCTTTGCTTTTCTGGCTCAGG | NS4A |
NS4B-F2 | TGAATTCGCCACCATGTCTCCCCAGGACAACCAAATG | NS4B |
NS4B-R2 | GCTCGAGACGTCTCTTGACCAAGCCAGC | NS4B |
NS5-F1 | TGAATTCGGGGGTGGAACAGGAGAGA | NS5 |
NS5-R1 | TCTCGAGTCACAGCACTCCAGGTGTAGAC | NS5 |
ZIKV-RR-F | AARTACACATACCARAACAAAGTGGT | NS5 |
ZIKV-RR-R | TCCRCTCCCYCTYTGGTCTTG | NS5 |
KPNA2-RR-F1 | TGCTGGGCTATTTCCTACCT | KPNA2 |
KPNA2-RR-R1 | GCTCTTAGGGCAGGAGTCAC | KPNA2 |
PR-NS2A-R3 | ACTCGAGTGTCCAATTAGCTCTGAAGATGAAAG | NS2A |
PR-NS2A-R4 | ACTCGAGAAACCCCCCGCAAGTAGCAAG | NS2A |
PR-NS2A-F3 | TGAATTCGCCATGCTGGTATCTTTCATCTTCAGAG | NS2A |
PR-NS2A-F4 | TGAATTCGCCATGCCACTGGCCCGGGGCACACTG | NS2A |
NS2A-R96A-F | TTCATCTTCGCAGCTAATTGGACACCCCGTGAAAGC | NS2A-M1 |
NS2A-R96A-R | TGTCCAATTAGCTGCGAAGATGAAAGATACCAGCAACG | NS2A-M1 |
NS2A-N98A-F | TCTTCAGAGCTGCTTGGACACCCCGTGAAAGCATGC | NS2A-M2 |
NS2A-N98A-R | CGGGGTGTCCAAGCAGCTCTGAAGATGAAAGATACCAG | NS2A-M2 |
NS2A-W99A-F | TCAGAGCTAATGCGACACCCCGTGAAAGCATGCTGC | NS2A-M3 |
NS2A-W99A-R | TCACGGGGTGTCGCATTAGCTCTGAAGATGAAAGATAC | NS2A-M3 |
NS2A-T100A-F | AGCTAATTGGGCACCCCGTGAAAGCATGCTGCTGGC | NS2A-M4 |
NS2A-T100A-R | GCTTTCACGGGGTGCCCAATTAGCTCTGAAGATGAAAG | NS2A-M4 |
KPNA2-F1 | CGAATTCGCCATGTCCACCAACGAGAATGCTAATA | KPNA2 |
KPNA2-R1 | GCTCGAGCTAAAAGTTAAAGGTCCCAGGAGCC | KPNA2 |
K2-LS-M-F1 | TACTCAAGCTGCCAGGAAACTAGCAGCAAGAGAAAAACAGCCCCCCATAG | KPNA2-M1 |
K2-LS-M-R1 | CTATGGGGGGCTGTTTTTCTCTTGCTGCTAGTTTCCTGGCAGCTTGAGTA | KPNA2-M1 |
K2-RK-M-F1 | AGCTGCCAGGAAACTACTTTCCGCAGAAGCACAGCCCCCCATAGACAACATAA | KPNA2-M2 |
K2-RK-M-R1 | TTATGTTGTCTATGGGGGGCTGTGCTTCTGCGGAAAGTAGTTTCCTGGCAGCT | KPNA2-M2 |
K2-E-M-F1 | TGCCAGGAAACTACTTTCCAGAGCAAAACAGCCCCCCATAGACAACA | KPNA2-M3 |
K2-E-M-R1 | TGTTGTCTATGGGGGGCTGTTTTGCTCTGGAAAGTAGTTTCCTGGCA | KPNA2-M3 |
K2-Q-M-F1 | GAAACTACTTTCCAGAGAAAAAGCGCCCCCCATAGACAACATAATCC | KPNA2-M4 |
K2-Q-M-R1 | GGATTATGTTGTCTATGGGGGGCGCTTTTTCTCTGGAAAGTAGTTTC | KPNA2-M4 |
LAMP2A-ShRNA1F | GATCCGCACCATCATGCTGGATATTTCAAGAGAATATCCAGCATGATGGTGCTTTTTTACGCGTG | shLAMP2A |
LAMP2A-ShRNA1R | AATTCACGCGTAAAAAAGCACCATCATGCTGGATATTCTCTTGAAATATCCAGCATGATGGTGCG | shLAMP2A |
LAMP2A-ShRNA2F | GATCCGCAGGAGTACTTATTCTAGTTCAAGAGACTAGAATAAGTACTCCTGCTTTTTTACGCGTG | shLAMP2A |
LAMP2A-ShRNA2R | AATTCACGCGTAAAAAAGCAGGAGTACTTATTCTAGTCTCTTGAACTAGAATAAGTACTCCTGCG | shLAMP2A |
KPNA2-shRNA1F | GATCCGCTTAATTGAGAAGTATTATCAAGAGATAATACTTCTCAATTAAGCTTTTTTACGCGTG | shKPNA2 |
KPNA2-shRNA1R | AATTCACGCGTAAAAAAGCTTAATTGAGAAGTATTATCTCTTGATAATACTTCTCAATTAAGCG | shKPNA2 |
KPNA2-shRNA2F | GATCCGTGTCAAAGGCATAAATAATCAAGAGATTATTTATGCCTTTGACACTTTTTTACGCGTG | shKPNA2 |
KPNA2-shRNA2R | AATTCACGCGTAAAAAAGTGTCAAAGGCATAAATAATCTCTTGATTATTTATGCCTTTGACACG | shKPNA2 |
ICD-NS2A-M-F1 | CACGTGGAGGAAACATGTGGAACAAGAGGA | NS2A |
ICD-NS2A-M-R1 | CCTCCACCAGGGAGAAATCACCACTCTCATCT | NS2A |
NS2A-ICD-SEQ-R | CATCAGGTCGCCTTCCAAGGCGGAGATCGC | NS2A |
aF: forward primer, R: reverse primer.
bThe italicized alphabets indicate restriction enzyme cleavage sites for cloning.