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. 2020 Sep 28;16(12):2238–2251. doi: 10.1080/15548627.2020.1823122

Table 1.

List of primers used in this study

Primera Sequences (5ʹ to 3ʹ)b Target gene/vector
C-F1 CGAATTCATGAAAAACCCAAAAAAGAAATC C
C-R2 GCTCGAGTGCCATGGCTGTGGTCAGCAG C
PRM-F2 TGAATTCGCCACCATGGGCACAGATACTAGTGTCGGAAT prM
PRM-R2 GCTCGAGGCTGTATGCCGGGGCAATCAG prM
E-F2 TGAATTCGCCACCATGCAAAAAGTCATATACTTGGTC E
E-R2 GCTCGAGAGCAGAGACGGCTGTGGATAAG E
NS2A-F2 TGAATTCCACCATGGGATCAACTGATCACATGGAC NS2A
SPG-C16-NS2A-F1 CGAATTCGCCACCATGGGAGTCAAAGTTCTGTTTGCCCTGATCTGCATCGCTGTGGCCGAGCCCAGGAAAGAACCAGAAAG NS2A
NS2A-R2 TCTCGAGCCGCTTCCCACTCCTTGTGAG NS2A
NS2B-F1 TGAATTCAGCTGGCCCCCTAGCGAAGTAC NS2B
NS2B-R1 GCTCGAGTCACCTTTTTCCAGTCTTCACG NS2B
NS3-F1 TGAATTCAGTGGTGCTCTATGGGATGTG NS3
NS3-R1 TCTCGAGTCATCTTTTCCCAGCGGCAAACTC NS3
NS4A-F1 TGAATTCGGAGCGGCTTTTGGAGTGATG NS4A
NS4A-R1 TCTCGAGTCATCTTTGCTTTTCTGGCTCAGG NS4A
NS4B-F2 TGAATTCGCCACCATGTCTCCCCAGGACAACCAAATG NS4B
NS4B-R2 GCTCGAGACGTCTCTTGACCAAGCCAGC NS4B
NS5-F1 TGAATTCGGGGGTGGAACAGGAGAGA NS5
NS5-R1 TCTCGAGTCACAGCACTCCAGGTGTAGAC NS5
ZIKV-RR-F AARTACACATACCARAACAAAGTGGT NS5
ZIKV-RR-R TCCRCTCCCYCTYTGGTCTTG NS5
KPNA2-RR-F1 TGCTGGGCTATTTCCTACCT KPNA2
KPNA2-RR-R1 GCTCTTAGGGCAGGAGTCAC KPNA2
PR-NS2A-R3 ACTCGAGTGTCCAATTAGCTCTGAAGATGAAAG NS2A
PR-NS2A-R4 ACTCGAGAAACCCCCCGCAAGTAGCAAG NS2A
PR-NS2A-F3 TGAATTCGCCATGCTGGTATCTTTCATCTTCAGAG NS2A
PR-NS2A-F4 TGAATTCGCCATGCCACTGGCCCGGGGCACACTG NS2A
NS2A-R96A-F TTCATCTTCGCAGCTAATTGGACACCCCGTGAAAGC NS2A-M1
NS2A-R96A-R TGTCCAATTAGCTGCGAAGATGAAAGATACCAGCAACG NS2A-M1
NS2A-N98A-F TCTTCAGAGCTGCTTGGACACCCCGTGAAAGCATGC NS2A-M2
NS2A-N98A-R CGGGGTGTCCAAGCAGCTCTGAAGATGAAAGATACCAG NS2A-M2
NS2A-W99A-F TCAGAGCTAATGCGACACCCCGTGAAAGCATGCTGC NS2A-M3
NS2A-W99A-R TCACGGGGTGTCGCATTAGCTCTGAAGATGAAAGATAC NS2A-M3
NS2A-T100A-F AGCTAATTGGGCACCCCGTGAAAGCATGCTGCTGGC NS2A-M4
NS2A-T100A-R GCTTTCACGGGGTGCCCAATTAGCTCTGAAGATGAAAG NS2A-M4
KPNA2-F1 CGAATTCGCCATGTCCACCAACGAGAATGCTAATA KPNA2
KPNA2-R1 GCTCGAGCTAAAAGTTAAAGGTCCCAGGAGCC KPNA2
K2-LS-M-F1 TACTCAAGCTGCCAGGAAACTAGCAGCAAGAGAAAAACAGCCCCCCATAG KPNA2-M1
K2-LS-M-R1 CTATGGGGGGCTGTTTTTCTCTTGCTGCTAGTTTCCTGGCAGCTTGAGTA KPNA2-M1
K2-RK-M-F1 AGCTGCCAGGAAACTACTTTCCGCAGAAGCACAGCCCCCCATAGACAACATAA KPNA2-M2
K2-RK-M-R1 TTATGTTGTCTATGGGGGGCTGTGCTTCTGCGGAAAGTAGTTTCCTGGCAGCT KPNA2-M2
K2-E-M-F1 TGCCAGGAAACTACTTTCCAGAGCAAAACAGCCCCCCATAGACAACA KPNA2-M3
K2-E-M-R1 TGTTGTCTATGGGGGGCTGTTTTGCTCTGGAAAGTAGTTTCCTGGCA KPNA2-M3
K2-Q-M-F1 GAAACTACTTTCCAGAGAAAAAGCGCCCCCCATAGACAACATAATCC KPNA2-M4
K2-Q-M-R1 GGATTATGTTGTCTATGGGGGGCGCTTTTTCTCTGGAAAGTAGTTTC KPNA2-M4
LAMP2A-ShRNA1F GATCCGCACCATCATGCTGGATATTTCAAGAGAATATCCAGCATGATGGTGCTTTTTTACGCGTG shLAMP2A
LAMP2A-ShRNA1R AATTCACGCGTAAAAAAGCACCATCATGCTGGATATTCTCTTGAAATATCCAGCATGATGGTGCG shLAMP2A
LAMP2A-ShRNA2F GATCCGCAGGAGTACTTATTCTAGTTCAAGAGACTAGAATAAGTACTCCTGCTTTTTTACGCGTG shLAMP2A
LAMP2A-ShRNA2R AATTCACGCGTAAAAAAGCAGGAGTACTTATTCTAGTCTCTTGAACTAGAATAAGTACTCCTGCG shLAMP2A
KPNA2-shRNA1F GATCCGCTTAATTGAGAAGTATTATCAAGAGATAATACTTCTCAATTAAGCTTTTTTACGCGTG shKPNA2
KPNA2-shRNA1R AATTCACGCGTAAAAAAGCTTAATTGAGAAGTATTATCTCTTGATAATACTTCTCAATTAAGCG shKPNA2
KPNA2-shRNA2F GATCCGTGTCAAAGGCATAAATAATCAAGAGATTATTTATGCCTTTGACACTTTTTTACGCGTG shKPNA2
KPNA2-shRNA2R AATTCACGCGTAAAAAAGTGTCAAAGGCATAAATAATCTCTTGATTATTTATGCCTTTGACACG shKPNA2
ICD-NS2A-M-F1 CACGTGGAGGAAACATGTGGAACAAGAGGA NS2A
ICD-NS2A-M-R1 CCTCCACCAGGGAGAAATCACCACTCTCATCT NS2A
NS2A-ICD-SEQ-R CATCAGGTCGCCTTCCAAGGCGGAGATCGC NS2A

aF: forward primer, R: reverse primer.

bThe italicized alphabets indicate restriction enzyme cleavage sites for cloning.