FIGURE 7.

Effect of CD-mediated autophagy on liver injury induced by APAP overdose in mice. Liver tissues were collected from mice 6 h after APAP challenge and analyzed by western blot. (A–F) Effects of CD on protein abundance of p62, p-mTOR, Beclin-1, Atg5, Atg7, LC3I, and LC3II measured by western blotting. (G–I) Effect of CD on phosphorylation of AMPK and nuclear translocation of TFEB. β-Actin or Lamin B1 was used as an internal control. Similar results were obtained from three independent experiments. All data are presented as means ± SEM (n = 10 in each group). *p < 0.05 and **p < 0.01 vs. control group; ^# p < 0.05 and ^## p < 0.01 vs. APAP group.