FIGURE 8.

NFE2L2 deficiency strengthened autophagy caused by CD treatment in mice with APAP-induced ALI. WT and NFE2L2^−/− mice were injected with CD (100 mg/kg) for 24 h followed by challenge with APAP (900 mg/kg). Liver tissue was collected 6 h after APAP challenge and analyzed by western blotting. (A–G) Effects of CD on protein abundance of Beclin-1, Atg5, Atg7, LC3, and phosphorylation of mTOR at serine 2,448 measured by western blot. β-Actin was used as an internal control. Similar results were obtained from three independent experiments. (H) Survival rate of mice within 24 h after APAP administration. All data are presented as means ± SEM (n = 10 in each group). *p < 0.05 and **p < 0.01 vs. WT control group; ^# p < 0.05 and ^## p < 0.01 vs. WT APAP group; ^† p < 0.05 and ^†† p < 0.01 vs. NFE2L2^−/− control group; ^§ p < 0.05 and ^§§ p < 0.01 vs. NFE2L2^−/− APAP group.