Figure 5.

Circ_0008450 silence inhibited the growth of keratinized epithelial cell via the up-regulation of Runx3. (a) Keratinized epithelial cells transfected with sh-Runx3 (sh-Runx3) or sh-control were applied for the detection of Runx3 mRNA by qRT-PCR. *** p < 0.001. Keratinized epithelial cells transfected with sh-circ_0008450 (sh-circ_0008450) or sh-Runx3 (sh-Runx3) or both were applied for the detection of cell proliferation and apoptosis. Proliferation marker (b) viability and (c) BrdU⁺ cells were assessed by CCK-8 assay and BrdU incorporation labeling respectively. ** p < 0.01 or *** p < 0.001. Apoptosis marker (d) apoptotic rate and (e and f) CyclinD1, CDK4, cleaved caspase 3 and cleaved caspase 9 proteins were assessed by apoptosis assay and western blot respectively. * p < 0.05, ** p < 0.01 or *** p < 0.001