Figure 4.

Overexpression of miR-877 promotes NSCLC cell apoptosis and cell cycle arrest. In the SPC-A-1 cells miR-877 was overexpressed and in the 95 C cells the miR-877 was inhibited. (a). Cell cycle distribution of SPC-A-1 cells and 95 C cells by flow cytometry; (b). Apoptosis rate of SPC-A-1 cells and 95 C cells by flow cytometry; (c). Representative images of morphological changes of apoptotic cells by Hoechst 33,258 staining; (d). Relative levels of apoptosis-related markers detected by western blot analysis. The experiment was performed three times independently. The results are presented as the mean ± standard deviation. Compared to the miR-NC group, * p < 0.05, ** p < 0.01. miR-877, microRNA-877; LC, lung cancer; NC, negative control