Figure 5.

Overexpression of miR-877 inhibits the EMT, cell migration and invasion of LC cells. In the SPC-A-1 cells miR-877 was overexpressed and in the 95 C cells the miR-877 was inhibited. (a). Relative mRNA expression of EMT markers; (b). Relative protein levels of EMT markers; (c). Relative E-cadherin positive cells and decreased vimentin in SPC-A-1 cells and 95 C cells by immunofluorescence assay; (d). Relative migration ability of SPC-A-1 cells and 95 C cells in vitro; (e). Relative invasive ability of SPC-A-1 cells and 95 C cells in vitro. The experiment was performed three times independently. The results are presented as the mean ± standard deviation. Compared to the miR-NC group, * p < 0.05, ** p < 0.01. miR-877, microRNA-877; LC, lung cancer; EMT, Epithelial-mesenchymal transition; NC, negative control