Figure 6.

miR-877 suppresses the growth, invasion and migration of NSCLC cells by decreasing ACP5. (a). Relative mRNA expression of ACP5 in SPC-A-1 cells and 95 C cells measured by qRT-PCR; (b). Relative protein level of ACP5 in SPC-A-1 cells and 95 C cells detected by western blot analysis; (c). Relative OD value detected by MTT assay; (d). Relative EdU positive ratio detected by EdU assay; (e). Relative colony formation ability of cells determined by colony formation assay; (f). Relative cell cycle distribution detected by flow cytometry; (g). Relative apoptosis rate detected using flow cytometry; H. Representative images of apoptotic cells measured by Hoechst 33,258 staining; (i). Relative mRNA expression of E-cadherin and N-cadherin measured by qRT-PCR; (j). Relative protein levels of E-cadherin and N-cadherin detected by western blot analysis; (k). Representative images of E-cadherin positive cells decreased and vimentin-positive cells obtained by immunofluorescent staining; (l). Relative migration ability of cells determined by scratch test; (m). relative invasion ability of cells determined by Transwell assay. The experiment was performed three times independently. The results are presented as the mean ± standard deviation. Compared to the miR-NC group, * p < 0.05, ** p < 0.01. miR-877, microRNA-877; LC, lung cancer; ACP5, tartrate resistant acid phosphatase 5; OD, optical density; EdU, 5-ethynyl-2ʹ-deoxyuridine; NC, negative control