Figure 5.

Curcumin suppresses protein synthesis, cell growth, and cell proliferation. (a) Global protein synthesis detected by SUnSET in HEK293 cells. The cells were cultured with or without insulin, and treated with curcumin or rapamycin. The specificity of the anti-puromycin (Puro) antibody was demonstrated by a sample without puromycin treatment. Coomassie blue staining was used as loading control. (b) Relative cell diameter of cells treated with curcumin or rapamycin. (c) Statistical analysis of average cell diameter of cells in (b). Data are shown as mean ± SEM; n = 500. **P < 0.01, ***P < 0.001. (d) HEK293 cells treated with or without curcumin. After 24 h, cells were subjected to BrdU incorporation assay. Scale bar: 50 µm. (e) Statistical analysis of the percentage of cells with BrdU incorporation in (d). Data are shown as mean ± SEM; n = 50. **P < 0.01