Figure 1.

Matrine suppresses lung CSCs properties

(a) The types of division is defined in Matrine-treated and not treated cells and is represented by white arrows. CSCs marker KLF4 and TCF4 were stained to illustrate symmetric division and asymmetric division of lung CSCs.(b) A549 and H460 sphere-forming cells were treated with various concentrations of Matrine in 0.6 mg/ml, 1.0 mg/ml, 1.4 mg/ml for 10 days (scale bar: 100 μm).(c) The protein levels of lung CSCs markers and key components of the Wnt signaling pathway were determined by Western blotting.