Figure 3.

Matrine/Let-7b/CCND1 suppresses CSCs in NSCLC

(a, b) A549 and H460 sphere-forming cells were treated with 5-FU and Matrine alone, or with Let-7b mimic or CCND1siRNA combination.(a) Representative pictures of Spheroids after 10 days of treatment accordingly.(b) CSCs biomarkers CD133 and KLF4 expressions were evaluated with immunoblotting.(c) A549 and H460 cells were treated with CCND1 upregulation inducer Tetracycline (TET) and Matrine alone or in combination.(d, e) Flow cytometric analysis of CD133-positive cells in A549 spheres-forming cells. Columns, mean (n = 3); bars, SD; *, p < 0.05