Figure 6.
Matrine repressed tumor proliferation in vivo
(a) Cells were subcutaneous injected in nude mice, which intraperitoneally administrated by vehicle, Matrine (20 mg/kg), 5-FU (20 mg/kg), or two drugs.(b) Tumor volume (mm3) was measured at every 5 treatment days.(c) Tumor weights were determined after the treatment was completed on day 30. Both Matrine and 5-FU significantly suppressed tumor volume and weight, while the combination therapy further enhanced the antitumor effect compared with a single therapy. Columns, mean (n = 3); bars, SD; *, p < 0.05(d) In the immunohistochemical analysis of tumor sections, Matrine could effectively inhibit CCND1 expression which was accumulated by 5-FU.