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. 2020 Nov 19;11:579265. doi: 10.3389/fphar.2020.579265

FIGURE 5.

FIGURE 5

Snail is involved in m6A-regulated EMT in renal epithelial cells. (A) HK2 cells were treated with genistein (15 μM) with or without TGF-β (5 ng/ml) for 24 h. The protein expressions of E-cadherin, α-SMA, and ALKBH5 were assayed by Western blot. (B) Quantification of (A) (n = 3, *p < 0.05, **p < 0.01 vs. control; #p < 0.05 vs. TGF-β group). (C) HK2 cells were treated with genistein (15 μM) and TGF-β (5 ng/ml) with the synthesized human ALKBH5 siRNA oligonucleotides and the scrambled oligonucleotides (siCon) at final concentrations of 50 nM. The protein expressions of ALKBH5, E-cadherin, α-SMA, and snail as revealed by Western blot. (D) Quantification of (C) (n = 3, *p < 0.05, **p < 0.01 vs. siCon). (E) HK2 cells were transfected with pcDNA/ALKBH5 or vector control through liposome-mediated transfection. The protein expressions of ALKBH5, E-cadherin, α-SMA, and snail as revealed by Western blot. (F) Quantification of (E) (n = 3, *p < 0.05, **p < 0.01 vs. vector). Data are presented as mean ± SD.