PKG activation in response to A, diethylenetriamine NONOate (DETA NONOate) and B, 8-para-chlorophenylthio-cGMP (8p-CPT-cGMP) in mesenteric vessels from vehicle (V), L-buthionine sulfoximine (BSO), tempol (T) and BSO + T -treated rats. C, PKG activation in response to DETA NONOate in presence and absence of sGC inhibitor ODQ (20 μM) and PKG inhibitor KT5823 (1 μM) in mesenteric tissue from vehicle treated rats. D, PKG-1α/β protein expression in mesenteric tissue homogenates. Bars represent mean ± SE from 6–8 rats performed in triplicate. *P<0.05 vs. respective basal, using 2-way ANOVA followed by post hoc Newman-Keuls multiple test.