A, TRPC4 expression under basal conditions in mesenteric vessels from vehicle (V), L-buthionine sulfoximine (BSO), tempol (T) and BSO + T -treated rats. B, mesenteric vessels were incubated with diethylenetriamine NONOate (DETA NONOate) followed by immunoprecipitation by VASP and phosphorylated VASP (P-VASP) antibodies. The immunoprecipitates were immunoblotted for TRPC4. Bars represent mean ± SE from 6–8 rats performed in triplicate. *P<0.05 vs. V and #P<0.05 vs. BSO, using 1-way ANOVA followed by post hoc Newman-Keuls multiple test.