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. 2012 Mar 21;18(1):805–815. doi: 10.2119/molmed.2011.00461

Figure 1.

Figure 1

Evodiamine increases phosphorylation of CaMKII, AMPK, ACC and eNOS in endothelial cells (ECs). (A–E) Bovine aortic ECs (BAECs) were treated with 1 µmol/L evidiamine (Evo) for up to 240 min, then examined by Western blot analysis for protein levels of phosphorylated CaMKII at Thr286, CaMKII protein, phosphorylated AMPK at Thr172, AMPK, phosphorylated ACC at Ser79, ACC, phosphorylated eNOS at Ser635 and Ser1179 and eNOS. (F) BAECs were treated with 10 µmol/L capsaicin (Cap) for 15 min, and then examined by Western blot analysis for protein levels of phosphorylated AMPK at Thr172, AMPK, phosphorylated eNOS at Ser635 and Ser1179, and eNOS. Data are mean ± SD from 5 independent experiments. *P < 0.05 versus nontreated group.