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. 2020 Dec 14;13:12829–12843. doi: 10.2147/OTT.S283786

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© 2020 Deng et al.

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Over-expression of HMGA2 could promote the migration in M0-Mφs and TAMs-Mφs. (A and B) Expression of TAMs markers (CD68, CD163, and CD204) were detected via real-time PCR and Western blot assay, respectively. (C) Expression of IL-10 and IL-12 were detected via real-time PCR. (D) Secretion of IL-10 and IL-12 in the supernatant were detected by ELISA assay. (E and F) M0-Mφs or TAMs-Mφs were seeded into the upper chamber, while HMGA2 over-expressed or down-regulated HepG2 cells were seeded into the lower chamber. All cells were cultured for 48 h. Migration in M0-Mφs and TAMs-Mφs were observed by transwell assay (scar bar = 100 μm). **p < 0.01 or *p < 0.05.