Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
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|---|---|---|---|---|---|---|---|
| Gene (D. melanogaster) | Pvf1 | FlyBase | FLYB: FBgn0030964 | NA | |||
| Gene (D. melanogaster) | Pvf2 | FlyBase | FLYB: FBgn0031888 | NA | |||
| Gene (D. melanogaster) | Pvf3 | FlyBase | FLYB: FBgn0085407 | NA | |||
| Gene (D. melanogaster) | Pvr | FlyBase | FLYB: FBgn0085407 | NA | |||
| Gene (D. melanogaster) | Pi3K21B | FlyBase | FLYB: FBgn0020622 | NA | |||
| Gene (D. melanogaster) | Pi3K92E | FlyBase | FLYB: FBgn0015279 | NA | |||
| Gene (D. melanogaster) | Akt1 | FlyBase | FLYB: FBgn0010379 | NA | |||
| Gene (D. melanogaster) | Tsc1 | FlyBase | FLYB: FBgn0026317 | NA | |||
| Gene (D. melanogaster) | Tsc2 | FlyBase | FLYB: FBgn0005198 | NA | |||
| Gene (D. melanogaster) | Pli | FlyBase | FLYB: FBgn0025574 | NA | |||
| Gene (D. melanogaster) | sls | FlyBase | FLYB: FBgn0086906 | NA | |||
| Gene (D. melanogaster) | geko | FlyBase | FLYB: FBgn0020300 | NA | |||
| Gene (D. melanogaster) | Pi3K59F | FlyBase | FLYB: FBgn0015277 | NA | |||
| Gene (D. melanogaster) | Pi3K68D | FlyBase | FLYB: FBgn0015278 | NA | |||
| Gene (D. melanogaster) | rl | FlyBase | FLYB: FBgn0003256 | NA | |||
| Gene (D. melanogaster) | InR | FlyBase | FLYB: FBgn0283499 | NA | |||
| Gene (D. melanogaster) | FASN1 | FlyBase | FLYB: FBgn0283427 | NA | |||
| Gene (D. melanogaster) | FASN3 | FlyBase | FLYB: FBgn0287184 | NA | |||
| Strain, strain background (D. melanogaster) | w1118 | Vienna Drosophila Resource Center | VDRC ID: 60000 | 1w[1118] | |||
| Genetic reagent (D. melanogaster) | UAS-pvf1i/UAS-pvf1-i1 | Vienna Drosophila Resource Center | VDRC ID: 102699 | Genotype: P{KK112211}VIE-260B | |||
| Genetic reagent (D. melanogaster) | UAS-pvf1-i2 | National Institute of Genetics | NIG Fly: 7103 R-2 | NA | |||
| Genetic reagent (D. melanogaster) | UAS-pvf2-i | Vienna Drosophila Resource Center | VDRC ID: 102072 | Genotype: P{KK110608}VIE-260B | |||
| Genetic reagent (D. melanogaster) | UAS-pvf3-i | Vienna Drosophila Resource Center | VDRC ID: 105008 | Genotype: P{KK112796}VIE-260B | |||
| Genetic reagent (D. melanogaster) | musts | Perrimon Lab stock | PMID:28739899 | Genotype: w[*]; P[w[+mC]=tub]P-Gal80{[ts]}10; P{w[+mC]}=Gal4-{Mef2.R}3 | |||
| Genetic reagent (D. melanogaster) | v-i | National Institute of Genetics | NIG Fly: 2115 R-1 | NA | |||
| Genetic reagent (D. melanogaster) | pli-troj-Gal4 | Bloomington Drosophila Stock Center | BDSC: 77693 | Genotype: y[1] w[*]; Mi{Trojan-GAL4.2}Pli[MI00302-TG4.2]/TM3, Sb[1] Ser[1] | |||
| Genetic reagent (D. melanogaster) | sls-troj-Gal4 | Bloomington Drosophila Stock Center | BDSC: 67495 | Genotype: y[1] w[*]; Mi{Trojan-GAL4.1}sls[MI10783-TG4.1]/TM3, Sb[1] Ser[1] | |||
| Genetic reagent (D. melanogaster) | geko-troj-Gal4 | Bloomington Drosophila Stock Center | BDSC: 66833 | Genotype: y[1] w[*]; Mi{Trojan-GAL4.1}geko[MI02663-TG4.1]/TM3, Sb[1] Ser[1] | |||
| Genetic reagent (D. melanogaster) | pvrDN | Bloomington Drosophila Stock Center | BDSC: 58430 | w[1118]; P{w[+mC]=UASp Pvr.DN}D1 | |||
| Genetic reagent (D. melanogaster) | ATts | Perrimon lab | FBrf0234453 | yw/w; tub-Gal80ts/Cyo; Lpp-Gal4/TM6BTby+ | |||
| Genetic reagent (D. melanogaster) | oenots | Bloomington Drosophila Stock Center | BDSC: 65700 | Genotype: P{w[+mC]=Desat1 GAL4.E800}4M, P{w[+mC]=tubP-GAL80[ts]}2 | |||
| Genetic reagent (D. melanogaster) | pvr-i | National Institute of Genetics | NIG-FLY: 8222 R-2 | NA | |||
| Transfected construct | NA | NA | NA | NA | |||
| Biological sample (D. melanogaster) | Adult Abdominal Cuticle | NA | NA | NA | |||
| Antibody | Anti-Pvr (Rat, Polyclonal) | Rosin et al., 2004 | PMID:15056618 | 1/400 | |||
| Antibody | Anti-Pvf1 (Rat, Polyclonal) | Rosin et al., 2004 | PMID:15056618 | 1/100 | |||
| Antibody | Anti-p4E-BP (Rabbit, monoclonal) | Cell Signaling | 2855S | 1/200 | |||
| Recombinant DNA reagent | NA | NA | NA | NA | |||
| Sequence-based reagent | FASN1 (primer) | FlyPrimerBank | PP32811 | For: ATTATTGACGCTGGCCTAAACC Rev: TGCTGCTCAGTCTCCGAGT |
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| Sequence-based reagent | FASN2 (primer) | FlyPrimerBank | PP1235 | For: ACATTGTGATCTCGGGACTTTC Rev:CGCTAAAGAACTTGTCGTCGAA |
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| Sequence-based reagent | FASN3 (primer) | FlyPrimerBank | PP17912 | For: CGCCGATGGCGTCATTTTAAT Rev:CTCCAAAGAAGGTTGCATCAAAC |
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| Sequence-based reagent | Pvf1 (primer) | FlyPrimerBank | PP26981 | For: AATCAACCGTGAGGAATGCAA Rev: GCACGCGGGCATATAGTAGT |
|||
| Sequence-based reagent | ACC (primer) | FlyPrimerBank | PP34306 | For: CGAGCGGGCCATTAGGTTT Rev: GCCATCTTGATGTATTCGGCAT |
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| Peptide, recombinant protein | NA | NA | NA | NA | |||
| Commercial assay or kit | Chromium Single Cell 3’ Library and Gel Bead Kit v2 | 10x Genomics | PN-120267 | NA | |||
| Commercial assay or kit | Chromium i7 Multiplex Kit | 10x Genomics | PN-120262 | NA | |||
| Commercial assay or kit | Chromium Single Cell A Chip Kit | 10x Genomics | PN-1000009 | NA | |||
| Commercial assay or kit | Nuclei Isolation Kit: Nuclei PURE Prep | Sigma-Aldrich | Cat# NUC201-1KT | NA | |||
| Chemical compound, drug | NA | NA | NA | NA | |||
| Software, algorithm | Seurat | Stuart et al., 2019 | PMID:31178118 | NA | |||
| Software, algorithm | Harmony | Korsunsky et al., 2019 | PMID:31740819 | NA | |||
| Software, algorithm | SoupX | Young and Behjati, 2018 | Biorxiv | doi: https://doi.org/10.1101/303727 | |||
| Software, algorithm | Biorender | https://biorender.com/ | NA | Biorender was utilized to make the schematic diagrams used in this study. | |||
| Other | DAPI (nuclear stain) | Vector Laboratories | Cat# H-1200 | Ready to use | |||
| Other | BODIPY | ThermoFischer | Cat# D3823 | 1/500 dilution of a 1 mg/ml stock in DEPC | |||
| Other | SyBr Green | Bio-Rad iQ SYBR Green Supermix | Cat# 1708880 | Working concentration: 1X | |||
| Other | Phalloidin | ThermoFischer | A12381 | 1/100 dilution of a 1 mg/ml stock in Methanol | |||