Table 1. The list of 28 differentially expressed proteins and their abundance and expression in varicocele group compared with the healthy fertile men group.
| Gene | Accession No. | Protein name | Healthy fertile group | Varicocele group | NSAF ratio | Expression | ||
|---|---|---|---|---|---|---|---|---|
| SC | Abund | SC | Abund | |||||
| CCT4 | 38455427 | T-complex protein 1 subunit delta isoform a | 2.7 | VL | 0 | - | 0.00 | Unique to fertile group |
| CF1 | 119392081 | Complement factor I preproprotein | 1.3 | VL | 0 | - | 0.00 | Unique to fertile group |
| CCT8 | 48762932 | T-complex protein 1 subunit theta isoform 1 | 5.7 | VL | 0 | - | 0.00 | Unique to fertile group |
| TCP1 | 57863257 | T-complex protein 1 subunit alpha isoform a | 2.0 | VL | 0 | - | 0.00 | Unique to fertile group |
| RUVBL1 | 4506753 | RuvB-like 1 | 8.3 | L | 0.3 | VL | 0.02 | UE |
| APOA2a | 4502149 | Apolipoprotein A-II preproprotein | 1.3 | VL | 0.2 | VL | 0.04 | UE |
| RUVBL2 | 5730023 | RuvB-like 2 | 7.7 | VL | 0.5 | VL | 0.09 | UE |
| EEF1G | 4503481 | Elongation factor 1-gamma | 10.3 | L | 2.8 | VL | 0.24 | UE |
| PGK2 | 31543397 | Phosphoglycerate kinase 2 | 25.3 | M | 8.3 | L | 0.29 | UE |
| CASP12 | 300360580 | Inactive caspase-12 | 21.7 | M | 7.7 | VL | 0.31 | UE |
| ACRa | 148613878 | Acrosin precursor | 10.7 | L | 3.2 | VL | 0.32 | UE |
| SEMG1 | 4506883 | Semenogelin-1 preproprotein | 698.3 | H | 395.7 | H | 0.46 | UE |
| HSPA2a | 13676857 | Heat shock related 70 protein 2 | 46.3 | M | 37.5 | M | 0.48 | UE |
| SEMG2 | 4506885 | Semenogelin-2 precursor | 1321.7 | H | 765.0 | H | 0.48 | UE |
| ACTG1 | 4501887 | Actin, cytoplasmic 2 | 52.3 | M | 91.5 | H | 1.73 | OE |
| PRDX1 | 4505591 | Peroxiredoxin-1 | 13.3 | L | 35.7 | M | 2.09 | OE |
| PRDX2a | 32189392 | Peroxiredoxin-2 | 11.3 | L | 32.2 | M | 2.46 | OE |
| ANXA1 | 4502101 | Annexin A1 | 7.7 | VL | 24.7 | M | 3.05 | OE |
| CD63 | 383872538 | CD63 antigen isoform D precursor | 3.0 | VL | 8.3 | L | 3.15 | OE |
| TGM4 | 156627577 | Protein-glutamine gamma-glutamyltransferase 4 | 31.3 | M | 181.8 | H | 3.46 | OE |
| RAB27B | 530414276 | Ras-related protein Rab-27B isoform X1 | 2.0 | VL | 9.5 | L | 4.03 | OE |
| HIST1H4A | 4504301 | Histone H4 | 1.7 | VL | 9.8 | L | 5.67 | OE |
| CBR1 | 4502599 | Carbonyl reductase [NADPH] 1 isoform 1 | 0.7 | VL | 4.7 | VL | 6.27 | OE |
| LAMA5 | 145309326 | Laminin subunit gamma-1 precursor | 2.0 | VL | 25.7 | M | 7.69 | OE |
| LCN15 | 578817458 | Lipocalin-15 isoform X1 | 0.7 | VL | 6.2 | VL | 8.21 | OE |
| GLO1 | 118402586 | Lactoylglutathione lyase | 1.3 | VL | 11.5 | L | 9.22 | OE |
| LAMC1 | 578835999 | Laminin subunit alpha-5 isoform X1 | 1.7 | VL | 36.5 | M | 12.90 | OE |
| PIGR | 530366266 | Polymeric immunoglobulin receptor isoform X1 | 0.3 | VL | 34.0 | M | 70.67 | OE |
SC: spectral count, Abund: abundance, NSAF: normalized spectral abundance factor, VL: very low, L: low, M: medium, H: high, UE: underexpressed, OE: overexpressed.
aThe proteins HSPA2, APOA2, PRDX2 and ACR were selected for validation by Western blotting.