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. 2020 Dec 21;253(1):3. doi: 10.1007/s00425-020-03516-w

Fig. 7.

Fig. 7

Effect of aluminum on EcDQD/SDH gene expression (a) and the concentrations of DQD/SDH substrates and products in roots of Eucalyptus camaldulensis (b). Relative mRNA levels and metabolite concentrations were determined after a 5-day exposure to 0 or 1.5 mM AlCl3 in nutrient solution (pH 4). The EcDQD/SDH4a and EcDQD/SDH4b expression levels were quantified as the total mRNA abundance for sequence variants a and b. The mRNA level in the control roots was defined as 1. The mRNA level of the aluminum-responsive gene EcMATE1 (accession number AB725912) was used as a positive control for the aluminum treatment. The EcActin gene served as an internal control. The citrate concentration was used as a positive control for the aluminum treatment. Data are presented as the mean ± SD (n = 6). Asterisks indicate a significant difference between treatments at ***P < 0.001 (Student’s t test)