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. 2020 Dec 21;10:22187. doi: 10.1038/s41598-020-79136-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of filament bundling in synthetic images. (A) Representative synthetic images of virtual cytoskeleton bundling. The images were built by adding filaments with constant intensity and length at random positions. To mimic bundles, the positions of the filaments on the X-coordinate were restricted to 1/20 for different percentages of the filaments (0–80%). (B) Mean intensity of the images. (C) Skewness of the intensity distribution. (D) CV of the intensity distribution. Significance was tested using the Tukey–Kramer test (P < 0.01). Different lowercase letters indicate significant differences. N = 20.