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. 2020 Dec 21;10:22187. doi: 10.1038/s41598-020-79136-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of actin filament bundling in vitro. (A) Representative confocal laser scanning microscopy images of in vitro ATTO 390-labeled actin filaments. Control images (left) and those with bundling due to the addition of MgCl₂ (right) are shown. Scale bar indicates 20 μm. (B) Skewness of the intensity distribution. (C) CV of intensity distribution. Significance was determined using the Mann–Whitney U-test. N = 30.