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. 2020 Dec 21;11:6422. doi: 10.1038/s41467-020-20222-z

Fig. 6. Mll1 antagonizes PcG-mediated silencing of stem cell genes.

Fig. 6

a mRNA expression of LGR5 and OLFM4 in control (dark gray), 6d doxycycline-induced shMLL1 human Ls174T cells (dark green), and shMLL1 Ls174T cells following 4-day Gsk126 treatment (light green), n = 4 independent experiments, two-tailed unpaired t-test, LGR5 *p = 0.014, **p = 0.0016, *p = 0.027; OLFM4 **p = 0.008, *p = 0.03. Data are presented as mean values ± SD. b ChIP for H3K4me3 (dark gray columns) and H3K27me3 (light gray columns) at the TSSs of LGR5 and OLFM4 (n = 3 independent experiments) in control, 11d doxycycline-induced shMLL1 human Ls174T cells, and shMLL1 Ls174T cells following 4-day Gsk126 treatment, represented as % input. Data are presented as mean values ± SD. c mRNA expression of ASCL2 and AXIN2 in control (dark gray), 6d doxycycline-induced shMLL1 human Ls174T cells (dark green), and shMLL1 Ls174T cells following 4-day Gsk126 treatment (light green), n = 6 independent experiments, two-tailed unpaired t-test. Data are presented as mean values ± SD. d ChIP for H3K4me3 (dark gray columns) and H3K27me3 (light gray columns) at the TSSs of ASCL2 and AXIN2 (n = 3 independent experiments) in control, 11d doxycycline-induced shMLL1 human Ls174T cells, and shMLL1 Ls174T cells following 4-day Gsk126 treatment, represented as % input. Data are presented as mean values ± SD. e Scheme of β-catenin/TCF4/MLL1-dependent regulation of LGR5 in colon cancer cells. MLL1 prevents PRC2-mediated gene silencing to sustain LGR5 expression. This is associated with the MLL1-dependent H3K4 tri-methylation at the LGR5 TSS. Ablation of MLL1 results in PRC2-mediated H3K27me3 and silencing of LGR5 expression. Source data are provided as a Source Data file.