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. 2020 Dec 8;11:603131. doi: 10.3389/fmicb.2020.603131

Figure 1.

Figure 1

Disruptor of telomeric silencing 1-like (DOT1L) expression and H3K79me2 levels are upregulated during avian leukosis virus subgroup J (ALV-J) infection in chicken macrophage cell line HD11. (A,B) Western blot (A) and intensity (B) for DOT1L protein and DOT1L-mediated H3K79me2 levels in HD11 cells at the indicated time points post infection with ALV-J (MOI 2). α-tubulin and total H3 were used as a loading control. (C) The relative DOT1L mRNA levels in HD11 cells at the indicated time points post infection with ALV-J, measured by quantitative real-time reverse transcription PCR (qRT-PCR). GAPDH was used as a normalizing control. (D) The relative ALV-J RNA level in HD11 cells at the indicated time points post infection, normalized to chicken GAPDH mRNA, was measured by qRT-PCR. All the data were shown as mean ± SD (error bars) from three independent experiments. ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001; unpaired, two-tailed Student’s t-test.