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. 2020 Dec 8;11:590266. doi: 10.3389/fimmu.2020.590266

Figure 4.

Figure 4

CK2α suppresses myeloid cell accumulation for increased Lm burdens in trans. Bone marrow (BM) chimera experiments. (A) Schematic of experiment and graphs summarizing % of CD45.2 neutrophils and Ly6C+ monocytes in blood of irradiated CD45.1 recipients at 6 weeks after reconstitution with 1:1 mixtures of WT CD45.1 plus CD45.2 WT or MϕCK2α− / − BM cells. The proportion of CD45.2+ cells is also shown for mice reconstituted with only CD45.2 MϕCK2α− / − donor cells. (B–E) Splenocytes were harvested from Lm infected CD45.1+BL/6 and CD45.1+ MϕCK2α− / − chimeras 2 dpi. (B) Total numbers of neutrophils, Ly6C+ monocytes, and F4/80+ macrophages in the mixed chimeras were enumerated by cell counts and flow cytometry. (C) Proportions of CD45.2+ neutrophils, Ly6C+ monocytes, and F4/80+ macrophages in the infected mixed chimeric animals. Surface expression of (D) MHCII and (E) fluorescence of pHrodo at 1 h after uptake were compared between CD45.1+ and CD45.2+ Ly6C+ monocytes from chimeric mice. Shown are ratios of the MFI for each marker in the CD45.2 vs CD45.1 cells. (F) Bacteria burdens (CFUs) in livers and spleens of the respective chimeric animals at 2 dpi with Lm. Figure panels (A, F) show data pooled from three experiments with four to five mice/group/experiment, a group of mice received only CD45.2 MϕCK2α− / − BM cells in the third experiment. Figure panels (B–E) are data pooled from three experiments, eight to ten mice/group.