Fig. 5.

a) FISH images using a (CAG)₆ probe labelled with Texas Red at the 5′ end in combination with immunofluorescence staining. To verify the co-localization of MBNL1 in ribonuclear inclusions, following the last post-hybridization wash, cells were stained sequentially with antibodies to MBNL1 and with goat anti-mouse antibody conjugated with Alexa Fluor 488. Nuclei were visualized with Hoechst 33,258 dye. Insets show zoomed-in images (scale bars 20 µm). b) Quantitative FISH analysis of total foci per cell and colocalized MBNL1 in foci. Results are presented as mean (±SD) of n = 3 experiments. Statistical significance was assessed from a two-tailed Student's t-test (**p < 0.01, *p < 0.05).