Table 2.

Kinetic analysis of compounds 1c, and 1j.

Inhibitor
1c				1j
Conc.	Vmax (mM/min)	KM (mM)	Ki (M)	Conc.	Vmax (mM/min)	KM (mM)	Ki (M)
2.5 μM	4.0 × 10−2	6.56	3.02 × 10−6	5.0 μM	2.7 × 10−2	5.47	5.85 × 10−6
5.0 μM	4.0 × 10−2	10.72	2.51 × 10−6	10.0 μM	2.7 × 10−2	9.48	4.51 × 10−6
10.0 μM	4.0 × 10−2	19.93	2.19 × 10−6	20.0 μM	2.7 × 10−2	21.26	3.22 × 10−6

Data are mean values of 1/V (inverse of the increase in absorbance at a wavelength of 475 nm per min (ΔA₄₇₅/min)), of three independent experiments conducted using different L-tyrosine concentrations. The Lineweaver-Burk plot equation is: 1/V = 1/V_max + K_M/V_max × 1/[S] and the modified Michaelis-Menten equation is 1/V_max = (1 + [I]/K_i) × 1/K_M, where V is the reaction rate, V_max is the maximum reaction rate, K_M is the Michaelis-Menten constant, [S] is substrate concentration, [I] is inhibitor concentration, and K_i is the inhibition constant.