FIGURE 5.

The effects of AT-I on suppressing tumorigenesis was mediated by TLR4. The shTLR4 plasmid was used to transfect breast cancer cells, and then western blot and ELISA assays were used to compare related proteins expression with untransfected cells after cells had been pre-treated in presence or absence AT-I for 48 h during LPS stimulation. (A, B) The expression of TLR4/NF-κB signaling pathway was detected by western blot assay. (C, D) The levels of TNF-α, IL-6 and IL-1β in the cell supernatants were measured by ELISA assay. Significant differences between different groups were indicated as *p < 0.05, **p < 0.01, ***p < 0.001, vs. the LPS treated control group, n = 3.