Fig. 2.

Downregulation of PERK expression and activity in 22q11.2DS iPSC-derived dopaminergic neurons. (a) Immunoblotting for ER stress sensors and their related proteins using dopaminergic neurons (Day 24). P-PERK: phosphorylated PERK; P-eIF2α: phosphorylated eIF2α; P-IRE1: phosphorylated IRE1. (b–i) Quantification of the PERK/β-actin, P-eIF2α/eIF2α, ATF4/β-actin, GADD34/β-actin, ATF6/β-actin, GRP78/β-actin, IRE1/β-actin, P-IRE1/IRE1 ratios. Immunoblots were independently performed four (b and d; control [n = 12], 22DS [n = 24]), three (f and h; control [n = 9], 22DS [n = 18]) or two times (c, e, g and i; control [n = 6], 22DS [n = 12]). The value of Control1 was set to 1. Each plot represents the value of each line. Bars represent means ± SEs. *p < 0.05, **p < 0.01, ***p < 0.001.