Figure 3.

BTN3A genes harbor an atypical but functional SXY module

(A, C–E) Luciferase reporter assays were performed in HEK293T cells co-transfected with the parental pGL3 backbone or the indicated BTN3A2 (A, D) or BTN3A1 (C, E) promoter constructs, and an empty (mock) vector, or a vector coding for NLRC5 (A, C) or CIITA (D, E). SX-13bp-CCAAT contains the BTN3A1 or BTN3A2 promoter region, as indicated, with S-, X-, and 13 bp downstream CCAAT-box; where indicated, the CCAAT-box was mutated. (B) Alignment of the proximal BTN3A1-3 promoter region containing S- and X-motifs highlighted in blue and a 13 bp downstream “Y” CCAAT-box highlighted in pink. Distances between motifs are indicated. (A and C–E) Data are expressed as fold transactivation as compared with the mock condition. Results represent mean ± SD of n = 3 (A and D) and n = 4 (C and E) technical replicates and are representative of at least two independent experiments. Statistical differences were determined by performing a two-way ANOVA followed by comparison of the SX-13bp-CCAAT condition to the others transfected with the same NLR and were corrected for multiple testing using the Holm-Sidak method. Only statistically significant differences are illustrated. ∗∗∗p < 0.001.