Figure 4.

S-box sequence and CCAAT-box position are important for NLRC5 transactivation

(A and B) Luciferase reporter assays were performed in HEK293T cells co-transfected with the parental pGL3 backbone or the indicated BTN3A2 promoter constructs, and a vector coding for NLRC5 or CIITA I, or an empty (mock) vector; mtSX indicates a scrambled S-box sequence (the CAACGTT sequence was substituted by CCAGAGT) (B). Data are expressed as fold transactivation as compared to the mock condition. Results represent mean ± SD of n = 3 technical replicates and are representative of at least three independent experiments. Statistical differences were determined by performing a two-way ANOVA followed by comparison of the indicated conditions and were corrected for multiple testing using the Holm-Sidak method. ∗∗p < 0.01; ∗∗∗p < 0.001; ns: not significant.