Figure 5.

NLRC5 directly binds to BTN3A promoter regions

Vectors encoding human wild-type NLRC5 (wt NLRC5) or Walker A mutant (mt NLRC5) were cotransfected with CD72 into HEK293T. Forty-eight hours later, CD72-positive cells were MACS enriched and chromatin prepared. Binding of NLRC5 to the indicated promoters was assessed by chromatin immunoprecipitation (ChIP) followed by quantitative PCR. Results are expressed as fold enrichment as compared with the mt NLRC5 condition. Results are depicted as the mean ± SD of n = 3 technical replicates and are representative of at least two independent experiments. Statistical differences were determined by performing a two-way ANOVA followed by comparison of the wt NLRC5 with mt NLRC5 transfection and were corrected for multiple testing using the Holm-Sidak method. ∗∗∗p < 0.001.