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. 2020 Dec 8;11:598592. doi: 10.3389/fphar.2020.598592

FIGURE 2.

FIGURE 2

The myosin inhibitor, blebbistatin, inhibited exosome release from microglial cells under the stimulation of LPS. The extracted exosomes were divided into those extracted from control microglial cells, microglial cells incubated with 1 mg/mL LPS for 24 h, and microglial cells added with 1 μM blebbistatin 0.5 h before the 24 h stimulation of LPS. (A) Nanoparticle tracking analysis for the concentration of exosomes. (B) Flow cytometry analysis for CD9-labeled positive rate, CD81-labeled positive rate, or control FITC-IgG-labeled positive rate. (C) The protein expression levels of CD9 and CD81 exosome marker proteins were examined by Western blot. Western blots were quantified. Nine independent experiments were analyzed. A histogram depicts the quantitative representation of CD9 and CD81 protein expression for each group. The data were averages ±SD, n = 9. **p < 0.01 vs. control microglial cells; ## p < 0.01 vs. microglial cells stimulated with LPS.